ISSN 2285-5785, ISSN CD-ROM 2285-5793, ISSN ONLINE 2285-5807, ISSN-L 2285-5785
 

RESEARCH ON MICROFLORA ASSOCIATED WITH ALFALFA SEEDS (IN VITRO)

Published in Scientific Papers. Series A. Agronomy, Vol. LXV, Issue 1
Written by Gabi Valentin CÎRSTEA, Elena Ştefania IVAN, Stelica CRISTEA

The aim of the research was to identify fungi and the presence of other microorganisms on alfalfa seeds. The genetic material was represented by seeds of the Dobrogea genotype. We worked with three variants in three repetitions, untreated seeds (control variant), seeds passed through sterile water and disinfected seeds with 70% ethanol solution variant, under controlled conditions. Observations were made on microorganisms present on alfalfa seeds, under controlled conditions. The incidence of fungal microorganisms that developed on the culture medium, PDA (potato-dextrose-agar) was determined. Among the fungi, pathogens were detected: Fusarium spp., Alternaria spp., Cladosporium herbarum, Aspergillus spp., Penicillium spp. The highest incidence in the case of fungi was calculated in Fusarium spp. and Aspergillus spp. In the sterile water variant (F = 22.2%). Penicillium spp and Fusarium spp pathogens were present in all variants studied.

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