Published in Scientific Papers. Series A. Agronomy, Vol. LXVII, Issue 1
Written by Floriana Maria STEFAN, Ioana BERINDEAN, Andreea TICAN, Ionuț RACZ
In order to optimize the potato breeding program in Romania, molecular research was developed so that we can have the way of expressing the genetic potential of the new breeding creations. This study aimed to establish a unique DNA fingerprint for two new Romanian potato cultivars from NIRDPSB Brasov, using RAPD molecular markers for genetic variability discrimination between them. Amplification was carried out with 17 arbitrary primers, but only 14 produced amplified sequences. OPC 08 produced the most banding patterns. The level of similarity between these two varieties of potato has approximately the same value, suggesting a stronger degree of relationship between varieties. Although the examined varieties have different genetic origins, their shared geographical origin and belonging to the same species, this can explain nearly identical similarity coefficient between them and to use of only two varieties for discrimination of genetic diversity can do a high degree of similarity. Discrimination between varieties was limited by the RAPD technique, we recommend using a large number of varieties or a more precise technique for improved accuracy.
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