Published in Scientific Papers. Series A. Agronomy, Vol. LXVII, Issue 1
Written by Ioan Alexandru SĂRĂCIN, Ionel OBRETIN, Emilia CONSTANTINESCU, Mihnea GLODEANU, Patricia-Aida SĂRĂCIN, Ion SĂRĂCIN
Antioxidant activity is a vital parameter in assessing the potential health benefits and practical applications of natural products. This study investigates the antioxidant potential of Amaranthus retroflexus L. extracts using the 2,2-diphenyl-1picrylhydrazyl (DPPH) method. The DPPH method, widely employed for its simplicity and reliability, relies on the ability of antioxidants to neutralize the purple-colored DPPH radical, leading to a color change in the solution. By establishing a concentration-response relationship through testing various concentrations of Amaranthus retroflexus L. extracts, a dose-response curve was constructed, pivotal for determining the IC50 value, which represents the concentration capable of scavenging 50% of free radicals. Lower IC50 values indicate stronger antioxidant activity. The findings reveal variations in the inhibitory potential of Amaranthus retroflexus L. extracts, suggesting their potential utility in pharmaceuticals, agriculture, and other industries. This study underscores the significance of exploring natural sources for antioxidant compounds and provides valuable insights into harnessing the antioxidant properties of Amaranthus retroflexus L. for various applications.
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