Published in Scientific Papers. Series A. Agronomy, Vol. LXV, Issue 2
Written by Andreea TICAN, Mihaela CIOLOCA, Carmen Liliana BĂDĂRĂU, Nina BĂRĂSCU, Monica POPA
In order to investigate the effect of osmotic agents on the induction of microtubers, a study was carried out in the Laboratory of Vegetable Tissue Cultures of NIRDPSB Brașov. Bifactorial experience included the following factors: experimental factor A, variety, with three graduations: a1 - Marvis; a2 - Castrum; a3 - Ervant (as a control, the average of the values obtained for the three varieties was taken into account) and experimental factor B, microtuberization medium (with 4 graduations): b1 - classical microtuberization medium without osmotic agent (control medium); b2 - microtuberization medium with mannitol; b3 - microtuberization medium with sorbitol; b4 - microtuberization medium with PEG. The determinations were made for number of microtuber/plantlets and weight of microtubers/plantlets, in function of experimental factors. Compared to the control medium, osmotic agents added to nutritive medium had a positive effect on studied parameters Therefore, osmotic agents in low concentrations can be introduced as a stimulator of microtuberization.
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