Published in Scientific Papers. Series A. Agronomy, Vol. LVIII
Written by Sebahattin ALBAYRAK, Mevlüt TÜRK, Cafer Sırrı SEVİMAY, Eissa ANAKHATOON
Alfalfa (Medicago sativa L.) is the one of the important and economic forage legume crops in the world and Turkey.Alfalfa has 60 species which 30 annual and perennial species were distributed in different regions of Turkey.Cytogenetical research on alfalfa lagged far behind other crops mainly because alfalfa chromosomes are very small,the chromosomes are morphologically very similar, cultivated alfalfa has relatively high number of chromosomes andalfalfa is an autotetraploid. The Yeşilova-3 alfalfa population was collected from Lake Regions of Turkey as clone. Roottips meristems obtained from plants of similar age that originated by vegetative reproduction from a single parent fromplants grown under greenhouse conditions, were pretreated with saturated solution of ά-bromonaphthalene and 0.002M 8-Hydroxiquinolin before staining with aceto-iron-hematoxylin. The measured chromosomal parameters were; Shortarm, Long arm, Total length of chromosomes, Arm Ratio and chromosome type. Within the chromotosome complement,seven pairs of chromosomes were metacentric (chromosomes II, III, V and VI) and three numbers were submetacentric(chromosome I, IV and VIII). Karyotype analysis showed that Yeşilova accession was tetraploid (2n=4x= 32). Theshort arm, long arm and total length of chromosomes were ranged from 0.82-1.22, 1.17-1.80 and 2.21-2.85micrometer, respectively.
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