ISSN 2285-5785, ISSN CD-ROM 2285-5793, ISSN ONLINE 2285-5807, ISSN-L 2285-5785


Published in Scientific Papers. Series A. Agonomy., Vol. LVI
Written by Mariana MARINESCU, Anca LĂCĂTUŞU, Mihail DUMITRU, Mihai MARINESCU

Crude oil bioremediation of soils is limited by the bacteria activity in degrading the spills hydrocarbons. Petroleum hydrocarbon pollution is one of the main environmental problems, not only by the important amounts released but also because of their toxicity. It is known that the main microorganisms consuming petroleum hydrocarbons are bacteria, so in this paper are presented the results obtained in a bioremediation laboratory experiment. The aim of this study is to enhance the bioremediation of soils polluted with crude oil by adding the natural biodegradable product and bacterial inoculum. A natural biodegradable product and bacterial inoculum was used for total petroleum hydrocarbon (TPH) removal from an artificial polluted soil. Soil polluted with 50000 mg/kg of TPH was treated with 0.25%, respective 0.5% and/or bacterial inoculum to increase the biodegradability rate. Also, the soil contaminated with 100000 mg/kg of TPH was treated with 0.5%, respective 1% and/or bacterial inoculum. The main objective of this work is to accelerate the biodegradation processes. The enhancement of petroleum hydrocarbons degradation was achieved under natural product treatment and bacterial inoculum. The bacterial inoculum was used to enrich indigenous microbes to enhance biodegradation rate in the green house experiment. In soil excessively polluted with crude oil, bacterial population size in conditioned variant with Ecosol maximum dose (1%) presented values comparable to those of inoculated variants, demonstrating the protective and stimulation effect of soil bacteria, including those involved in the degradation of petroleum hydrocarbons exercised by organic compound applied Ecosol.At each phase of the study, the natural biodegradable product was found to significantly enhance the biodegradation of petroleum hydrocarbons.

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