Published in Scientific Papers. Series A. Agronomy, Vol. LXIV, Issue 1
Written by Boyka MALCHEVA
Vegetation experiment with Lolium perenne L. was carried out on reclaimed substrates from humus depot, tailings pond and mine, as applied vermiculite, mineral fertilization and liming. Microbial biomass nitrogen shows the highest values after the addition of 10% vermiculite, simultaneous application of fertilization and liming and self-fertilization, compared to controls, without ameliorants. Activities of the studied enzymes urease and protease follow the increase of the concentration of vermiculite, they are also higher in the samples with self-fertilization and the combined application of fertilization and liming. The values of microbial biomass nitrogen and enzymatic activities are highest in the variants with substrates from the mine. Microbial nitrogen biomass strongly correlates with urease activity and moderately with protease activity.
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