Published in Scientific Papers. Series A. Agronomy, Vol. LXIII, Issue 1
Written by Yonko YONCHEV, Neli KERANOVA, Marina DRUMEVA-YONCHEVA
The subject of study in the present work is the spread of TEV from the potvirus group in Virginia, Burley and Basmi tobacco varieties. The aim is to monitor the occurrence and development of TEV in these varieties grown in the central parts of southern Bulgaria and to analyze their resistance to this virus. During the period 2010 - 2019 immunological studies were carried out to prove the new viral tobacco disease in Bulgaria. A serological identification kit of the French company SEDIAG S.A.S. was used to demonstrate the virus. To establish TEV, a TAS-ELISA was performed at 1: 100 dilution of IgG. The virus was found to be sporadic. From the eight years of the study, TEV has been proven in four. The 2010-2011 period is characterized by a significant prevalence of TEV in Burley Group tobacco (14.3% and 12.7%, respectively). The virus is most strongly developed in 2019. It is evidenced in 60% of the tested samples from the Virginia variety. During the eight-year study period, only in 2010 TEV was found in isolates from the three tobacco varieties - Virginia, Burley and Basmi. As a result of the single-factor analysis of variance and Duncan's test, it was proven that tobacco from the Basmi variety was the most susceptible to infection with the TEV virus (0.165), followed by Virginia (0.136). Burley tobacco was found most resistant to the disease of TEV (0.126).
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