Published in Scientific Papers. Series A. Agronomy, Vol. LXI, Issue 2
Written by Mihaela DUMITRU, Cristina TABUC, Ștefana JURCOANE
The aim of the study was to isolate, identify and preliminary characterize strains of Lactobacillus spp. from the gastrointestinal tract of piglets. Three isolated strains were assessed morphologically, culturally, biochemically and enzymatically (amylase, celullase, protease). Successive replications were performed on MRS + CaCO3 medium. The identification and analysis of the biochemical characteristics was performed by oxidase, catalase and API 50 CHL tests. From the all isolates, the Lactobacillus plantarum strain presents some potentially probiotic characteristics. The ability to synthesize organic acids (lactic, phenyl-lactic, hydroxyphenyl-lactic, acetic and propionic) was tested as well. The development of Lactobacillus plantarum was assessed on 4 variants of culture media containing different concentrations of ingredients (corn, soybean meal, substitute of milk). It was selected the first variant when was obtained 1.05 x1010 CFU/ml of L. plantarum. The feed additive was based on LABs, by following the biotechnological process steps. The procedure was performed in the biotechnological installation (IL 00). Subsequently, the optimum level of feed additive will be established in weaned piglet’s diet.
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